This elementary divergence in the active web-sites of NagA from these sources indicates that the system for that activation of your hydrolytic water and substrate essential for catalysis have to differ to a big diploma.

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Mutation of Y223 to some phenylalanine residue resulted inside of a mutant with practically the exact same catalytic action because the wild form enzyme and therefore this residue isn't considerable with the binding of substrate in NagA.

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